Blinded evaluation of DNA extraction and genotyping of stained Cryptosporidium on glass slides

نویسندگان
چکیده

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منابع مشابه

Extraction and genotyping of Cryptosporidium parvum DNA from fecal smears on glass slides stained conventionally for direct microscope examination.

A method was developed for extracting cryptosporidial DNA from stained fecal smears on glass microscope slides. The correct genotype of Cryptosporidium parvum was amplified by PCR from 89 (85%) of 105 smears following conventional staining but not from negative controls. This technique may have applications for analysis of other infectious agents.

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ژورنال

عنوان ژورنال: Letters in Applied Microbiology

سال: 2002

ISSN: 0266-8254,1365-2673

DOI: 10.1046/j.1472-765x.2002.01220.x